ABSTRACT
The aim of this study was to report the ability of killer toxins, previously used as biotyping techniques, as a new tool to differentiate C. albicans from C. dubliniensis. The susceptibility of C. albicans and C. dubliniensis to killer toxins ranged from 33.9 to 93.3 percent and from 6.67 to 93.3 percent, respectively.
Avaliou-se a capacidade das toxinas killer, previamente utilizadas na biotipagem de C. albicans, como método para diferenciar C. albicans de C. dubliniensis. A susceptibilidade de C. albicans e C. dubliniensis às toxinas killer variou de 33,9 por cento a 93,3 por cento para C. albicans e de 6,67 por cento a 93,3 por cento para C. dubliniensis.
Subject(s)
Candida/classification , Candida/drug effects , Cytotoxins/pharmacology , Killer Factors, Yeast/pharmacology , Mycological Typing Techniques/methods , Candida albicans/drug effects , Microbial Sensitivity TestsABSTRACT
Cerebral phaeohyphomycosis ("chromoblastomycosis") is a rare intracranial lesion. We report the first human culture-proven case of brain abscesses due to Fonsecaea pedrosoi in Brazil. The patient, a 28 year-old immunocompetent white male, had ocular manifestations and a hypertensive intracranial syndrome. Magnetic resonance imaging (MRI) of the brain revealed a main tumoral mass involving the right temporo-occipital area and another smaller apparently healed lesion at the left occipital lobe. A cerebral biopsy was performed and the pathological report was cerebral chromoblastomycosis. The main lesion was enucleated surgically and culture of the necrotic and suppurative mass grew a fungus identified as Fonsecaea pedrosoi. The patient had received a knife wound sixteen years prior to his hospitalization and, more recently, manifested a pulmonary granulomatous lesion in the right lung with a single non-pigmented form of a fungus present. It was speculated that the fungus might have gained entrance to the host through the skin lesion, although a primary respiratory lesion was not excluded. The patient was discharged from the hospital still with ocular manifestations and on antimycotic therapy and was followed for eight months without disease recurrence. Few months after he had complications of the previous neuro-surgery and died. A complete autopsy was performed and no residual fungal disease was found
Subject(s)
Humans , Male , Adult , Brain Abscess , Central Nervous System Fungal Infections , Chromoblastomycosis , Mitosporic Fungi , Brain Abscess , Central Nervous System Fungal Infections , Chromoblastomycosis , Fatal Outcome , Magnetic Resonance ImagingABSTRACT
Paciente portador de leucemia mieloide cronica, com irma HLA-compativel foi submetido a transplante alogenico de medula ossea. No dia +90 pos-TMO foi diagnosticado doenca do enxerto contra o hospedeiro (DECH) extensa e iniciado protocolo alternado de imunossupressao com altas doses de ciclosporina A e prednisona. O seguimento ambulatorial foi complicado, com granulocitopenia intermitente e quadros frequentes de sinusite e pneumonia. Um ano apos o transplante, o paciente apresentou rouquidao e voz anasalada. Foi realizada uma biopsia de corda vocal mas nenhum agente infeccioso pode ser identificado. Na diminuicao das doses das drogas imunossupressoras, houve piora da DECH cronica e foi reiniciado esquema de doses altas no dia +550. Tres meses apos, permanecendo o quadro de DECH fora do controle, foi tentado imunossupressao com azatioprina sem sucesso...
Subject(s)
Humans , Male , Adult , Fungi/isolation & purification , Sinusitis/complications , Bone Marrow Transplantation/adverse effects , Cyclosporine/therapeutic use , Graft vs Host Disease/complications , Graft vs Host Disease/therapy , Follow-Up Studies , Fungi/classification , Immunosuppression Therapy/methods , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/etiology , Maxillary Sinus/surgery , Respiratory Insufficiency/complicationsABSTRACT
O presente estudo trata dos resultados obtidos, do ponto de vista micologico e imunoquimico, de duas amostras de Paracoccidioides consideradas como pertencentes a espécie cerebriformis, criada por MOORE em 1935 e mantidas desde aquela época, através de repiques em agar-Sabouraud, na Micoteca do Instituto de Medicina Tropical de Säo Paulo. Após cerca de 60 anos, tais amostras conservavam as mesmas características descritas por MOORE (1935). Näo foram registradas lesöes experimentais em cobaias inoculadas por via intratesticular, näo se demonstrando também, pelas técnicas SDS PAGE e Western blotting...
Subject(s)
Immunoelectrophoresis , Paracoccidioides/classification , Paracoccidioidomycosis/virology , Blotting, Western , Culture Media , Immunohistochemistry , Microscopy, Electron, Scanning Transmission , Paracoccidioides/virologyABSTRACT
Os autores estudaram a prevalência da flora fúngica encontrada no pronto-socorro e unidades de tratamento intensivo dos Serviços de Gastroenterologia e Pneumologia do Hospital de Clínicas da Faculdade de Medicina da Universidade de Säo Paulo